Scanning electron micrograph shows a colony of Salmonella typhimurium bacteria. Photo courtesy of CDC, Janice Haney Carr

State of Salmonella: Technology and Industry Trying to Reduce Rates

By Maria Fontanazza
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Scanning electron micrograph shows a colony of Salmonella typhimurium bacteria. Photo courtesy of CDC, Janice Haney Carr

From poultry to fresh produce, fighting Salmonella contamination is requiring more federal measures and innovative technology for rapid detection.

By 2020, the CDC has targeted a reduction in Salmonella cases at 11.4 per 100,000 people (last year’s rate was 15.4 per 100,000). Considering that the number of cases has not declined in the past 10+ years, regulators and industry will need to work together more closely on utilizing methods and technologies that provide effective pathogen detection.

Scanning electron micrograph shows a colony of Salmonella typhimurium bacteria. Photo courtesy of CDC, Janice Haney Carr
Scanning electron micrograph shows a colony of Salmonella typhimurium bacteria. Photo courtesy of CDC, Janice Haney Carr

It is a well-known fact within the food safety industry that Salmonella is the second most common foodborne illness in the United States, and the bacteria comes in first place in the category of foodborne illnesses that lead to hospitalization or death.  Unfortunately, there has been no change in the number of confirmed infections between the 2006-2008 period and last year, according to the CDC’s Foodborne Diseases Active Surveillance Network (FoodNet) 2014 Food Safety Progress Report

“The major threat Salmonella poses is that its public health impact has remained largely unchanged despite many years of regulatory oversight and technological improvements,” says Evan Chaney, Ph.D., senior manager, scientific affairs at Roka Bioscience. “Reducing the existing public health burden of Salmonella is a complex challenge that will require new and innovative thought, research, and technological developments or processes.”

Each year Salmonella makes its way into the food supply through a variety of sources, sickening more than 1 million people in the United States and costing the country nearly $3.7 billion, according to the USDA. Although it is most commonly found in contaminated animal-derived products, the level of serious outbreaks over the past few years is cause for concern, as the bacteria have appeared in processed foods such as peanut butter and fresh produce, including cucumbers, cantaloupes, alfalfa sprouts and jalapeno peppers.

USDA’s Food Safety and Inspection Service (FSIS) proposed federal measures in January to lower the rate of Salmonella in poultry, including a pathogen reduction performance standard for chicken parts, and ground chicken and turkey. FSIS began the sample project for raw chicken parts in March (only for firms that produce more than 1,000 lbs of chicken parts daily) and a verification testing program for poultry carcass inspection, using a moving window of sampling results, in May (view the full Federal Register notice).

Technologies Taking Down Salmonella

Providing rapid pathogen detection for Salmonella in less than 24 hours has been a priority for industry. Companies want to both prevent contaminated foods from entering the market as well as ensure that products can be quickly tested so that they are not sitting in a warehouse for days awaiting results. With this focus comes a need to provide testing in the processing environment.

“I think we will see an increase in testing related to the Food Safety Modernization Act, and there’s a trend toward environmental testing as opposed to finished product testing,” says Meredith Sutzko, Product Manager, Food Pathogens of Romer Labs North America. “Processors are looking to identify contamination very early in the process. To do that, they need simple and cost-effective methods, especially ones that will deliver a fast time-to-result and can be used at the production facility so they don’t have to wait to obtain test results.”

From enzyme immunoassays (EIA) and enzyme-linked immunosorbent assays (ELISA) to PCR assays, there are a large variety of established testing and identification technologies on the market. However, in taking a different approach to lowering the risk of Salmonella from entering the market, companies can employ limits testing as an additional tool to verify process control within a production shift. Salmonella limits testing is a semi-quantitative method that provides a “positive” or “no-go” result based on initial contamination levels of a sample, and does so within a day.

Limits testing provides a semi-quantitative approach to lowering Salmonella levels.   Image courtesy of Roka Bioscience
Limits testing provides a semi-quantitative approach to lowering Salmonella levels.
Image courtesy of Roka Bioscience

“A key difference in this testing approach is the utilization of a pathogen test as a means of process control or intervention, an example of a tool utilized in a new manner,” says Chaney, adding that in most cases, pathogen testing is conducted on finished product or on lots of product in concordance with COA requirements. While it is important to provide results that indicate both qualitative (presence or absence) and quantitative (estimates concentration), having insight into actual concentration levels provides another layer of risk mitigation. “We need tools to help us understand what Salmonella loads are in the system in a rapid manner so that action can be taken. This is the purpose of limits testing.”

As a case example of limits testing being used in the field, Cargill asked Roka Bioscience to develop a limits-based approach for testing its ground turkey for Salmonella. Roka was required to provide Salmonella detection levels at about 1 CFU/g in a 375 g sample size of ground meat in less than eight hours (total time to result). Roka successfully developed the limits application, and Cargill now uses the tool to make product disposition decisions prior to shipping its ground turkey. Roka is also looking at other areas in which limits testing can be used, from the pre-harvest to live production environments.

Calling on industry to push harder to innovate in foodborne pathogen detection, FDA announced its 2014 Food Safety Challenge last fall. The agency is offering a hefty prize—$500,00—to the team that can develop a technique or technology that creates “significant improvements” in the speed at which FDA can detect Salmonella in fresh, minimally processed produce. The finalists, announced last month, have received $20,000 to further develop their concepts and are being mentored by FDA in food safety and pathogen testing.

Technology contenders for the challenge include:

  • A handheld magnetoelastic biosensor and surface-scanning detector that can be passed over food to detect surface contamination. The portable device can be used both in a process facility as well as remote locations in which produce is being harvested and delivers results in minutes, according to the team’s leader, Bryan Chin, director of the Auburn University Detection and Food Safety Center.
  • Patented high-throughput technology for capturing Salmonella in large sample volumes. Developed by UC Davis School of Veterinary Medicine and scientists from Mars, Inc., the test uses fluidized bed technology to detect bacteria in less than four hours and presents potential for larger food processors. View the UC Davis video
  • DNA aptamer-magnetic bead sandwich assay, for use with a handheld florescence reader. Developers of the assay are from Pronucleotein, Inc. and Xgenex
  • Method for concentrating salmonella to detectable levels using automated microfiltration, developed by Purdue University.
  • Portable system for multiplexed detection of foodborne pathogens in microfluidic biochips through isothermal DNA amplification and electrical detection, developed by University of Illinois and Purdue University.

On July 7, FDA is holding a Demo Day at the Center for Food Safety and Applied Nutrition in Maryland where the finalists will present their refined concepts to judges from the FDA, USDA, and CDC, and a live audience. The winner of the Food Safety Challenge will also be announced sometime next month.

About The Author

Maria Fontanazza, Food Safety Tech

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