The AOAC Research Institute approved a method extension of Performance Test Method #030502 to include the DuPont BAX System X5 PCR Assay for Genus Listeria. The assay has been validated as a reliable method for detecting the pathogen in frankfurters, smoked salmon, spinach, and cheese, as well as from environmental surfaces. Using the system, automated PCR-based DNA amplification and automatic pathogen detection can be conducted on 32 samples simultaneously within a smaller and more lightweight unit. The system is also validated by AOAC-RI for detecting Salmonella and Listeria monocytogenes in good and environmental samples.