Tag Archives: spectroscopy

Honey in spoon

Ensuring honey authenticity: the role of NMR spectroscopy

By Léa Heintz
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Honey in spoon

Honey is a key component in many households, valued for its health benefits, antibacterial, and antioxidant properties. However, its premium price point and resource-intensive production has led to the rise of honey fraud – the intentional adulteration of honey. This poses a significant challenge to the global honey industry by impacting beekeepers’ livelihoods, who cannot compete with cheaper adulterated alternatives, and damaging consumer trust.[i]

Traditional testing methods, which are focused on the detection of only one or a few parameters cannot reliably detect counterfeit honey products due to fraudsters finding new methods to bypass the tests. Nuclear magnetic resonance (NMR) spectroscopy has proven effective at uncovering a wide range of adulteration methods thanks to the multitude of parameters tested.

The impact and implications of honey adulteration

Economically motivated adulteration (EMA) is the intentional act of adulterating food for financial gain.

Some examples of EMA include the deliberate mislabeling of honey origin or variety and the addition of foreign sugars to the honey. The difficulty in detecting honey manipulation and the potential for economic gain provide attractive fraud opportunities for dishonest business operators.[ii]

A significant amount of imported honey is suspected to be adulterated and falsely labelled and goes undetected in the European market.[iii] There has been a significant rise in cases of honey adulteration in the EU in recent years. In 2017, more than 14 percent of tested honey samples had been adulterated;[iv] and, in 2023, 46 percent of honey samples were suspected of being adulterated with syrups.[v] The increased rates of honey adulteration could be attributed to the challenge of monitoring and keeping pace with evolving fraudulent practices. Consequently, non-targeted and multi-marker methods, which are not specific to a particular type of adulterant, are being increasingly adopted.

Current testing methods

There are several testing methods that currently exist to detect sugar syrups in honey. These methods involve detecting foreign enzymes that convert starches into sugars or specific markers present in syrups. However, there is evidence that these methods can be bypassed by fraudsters to allow adulterated honey to continue being undetectable.

Stable carbon isotope ratio analysis (SCIRA) can detect corn and sugar cane derived syrups. However, it is unable to detect other adulterants, such as syrups derived from C3 plants (for example beet, rice or wheat sugar).[vi] This is a significant limitation as fraudsters are able to bypass testing by adulterating using C3 sugars.

Another testing method is liquid chromatography-high resolution mass spectrometry (LC-HRMS) which is used for marker detection. However, its targeted approach means it can only detect known syrups, which have been measured before.

NMR: an effective tool for honey analysis

NMR is a rapid screening method which can detect signs of adulteration and other potential manipulations of honey. It provides a molecular ‘fingerprint’ of a honey sample, giving definitive information about its molecular composition and the presence of adulterants, such as foreign sugars.

NMR has the capacity to determine the country of origin and botanical source of honey[vii] which is useful for identification and detection of adulteration, and to ensure quality control.[viii] Other benefits of NMR analysis include its speed, reproducibility and the fact that sample preparation is very simple.

Thanks to the multiparametric approach offered by NMR, and its potential to uncover new markers, masking adulteration and misleading the analysis is difficult and its high reproducibility allows precise sample matching against databases of authenticated samples.

Case study: Estonia introduces measures to protect the honey industry

In 2019, the Estonian government declared NMR as the official testing method of honey products in Estonia.

Estonian beekeepers that were ethically producing authentic honey were unable to compete with the price of adulterated honey, causing them to lose business. In support of beekeepers, the Estonian government introduced measures to protect the honey industry.

This support has had a positive impact on the Estonian beekeeping industry, with producers able to sell their products at a reasonable price, allowing them to maintain their beehives and livelihoods.

The government collaborated with local beekeepers, food testing laboratories, honey packagers and retailers to remove fake honeys from the local market, including both locally sourced and imported products. The adoption of NMR testing could help ensure standards are met and address the widespread issue of honey fraud.

A bright future for the honey industry

Food fraud continues to be a global issue. Fraudsters are constantly finding new methods to bypass existing testing methods, which affects food production worldwide. In the case of honey, despite some governments implementing measures to prevent false labelling and protect the livelihoods of beekeepers, the lack of standardized regulations across different geographical regions allows counterfeit honey to avoid detection. NMR proves to be a highly reliable alternative to traditional testing methods. By providing detailed insight into a sample’s molecular composition, NMR is playing a role in safeguarding the honey industry and ensuring its sustainability.

References

[i] European Commission. “Official Controls: Food Fraud – Honey, Questions and Answers.” 2021. https://food.ec.europa.eu/document/download/7186ec16-8f9d-4459-b155-f424ee6c7e3e_en?filename=official-controls_food-fraud_2021-2_honey_qandas_en_0.pdf.

[ii] European Commission. “Official Controls: Food Fraud – Honey, Questions and Answers.” 2021. https://food.ec.europa.eu/document/download/7186ec16-8f9d-4459-b155-f424ee6c7e3e_en?filename=official-controls_food-fraud_2021-2_honey_qandas_en_0.pdf.

[iii] European Commission. “Honey: 2021-2022.” https://food.ec.europa.eu/safety/eu-agri-food-fraud-network/eu-coordinated-actions/honey-2021-2022_en.

[iv] European Commission. “Honey: 2015-2017.” https://food.ec.europa.eu/safety/eu-agri-food-fraud-network/eu-coordinated-actions/honey-2015-17_en.

[v] EU Coordinated Action. “From the Hives” (Honey 2021-2022). Food Safety. March 22, 2023. Accessed November 22, 2023. https://food.ec.europa.eu/safety/eu-agri-food-fraud-network/eu-coordinated-actions/honey-2021-2022_en.

[vi] Mai, Z.; Lai, B.; Sun, M.; Shao, J.; Guo, L. Food Adulteration and Traceability Tests Using Stable Carbon Isotope Technologies. Tropical Journal of Pharmaceutical Research 2019, 18 (8), 1771–1784.

[viii] Schepartz, A. I., & Subers, M. H. “Honey Composition and Properties.” Journal of Food Science and Technology (2019). https://www.tandfonline.com/doi/full/10.1080/87559129.2019.1636063#d1e891.

Olga Pawluczyk, P&P Optica

Ask the Expert: Olga Pawluczyk Discusses Hyperspectral Imaging

Olga Pawluczyk, P&P Optica

Can you explain, in simple terms, what hyperspectral imaging is?

Olga Pawluczyk: Hyperspectral imaging is a form of spectroscopy, which is the science of how wavelengths of light (or really, electromagnetic spectrum) interact with substances. As different wavelengths are absorbed by atomic and molecular bonds, we can measure that interaction and determine the chemistry of the substance under investigation. Essentially, your eyes and brain form a simple 3 color spectrometer: since you see grass as green, you can guess that it contains chlorophyll. Now, hyperspectral images include full 2D spatial information (like a regular camera image) but split the light into hundreds of continuous colors (or wavelengths). Compare this to the three colors (red, green, blue) used by cameras like the one in your cell-phone. Hyperspectral imaging allows much greater precision than other types of spectroscopy.

Why is hyperspectral imaging so effective for finding foreign (FM) materials in food products?

Pawluczyk: Using hyperspectral imaging, a system can see full images of objects and chemical signatures of different materials within those images. That’s what makes this technology so much better than other forms of vision or spectroscopy for distinguishing materials such as clear plastics, rubber and bone that are often hard to see on the line. Not only do we see the chemistry, but can also distinguish very small objects that differ in their chemistry from their surroundings. We can do this on line, at line speeds. PPO’s hyperspectral imaging system has been developed specifically for food processing, with rigorous testing and unique spectrometer design that allows us to see a lot of chemical information, while still enabling producers to run their lines at full speeds.Combining this with our powerful artificial intelligence (AI) engine makes our system uniquely effective at line speed, meaning contaminants can be identified and removed immediately.

What are the advantages of hyperspectral imaging over other types of detection systems?

Pawluczyk: In addition to being highly effective at finding FM, an important advantage of hyperspectral imaging is that it also enables us to see the composition of food products. Since food is chemistry, we can use hyperspectral imaging to assess different chemical properties of food products. For example, our testing has shown that spinach grown in different parts of the same field will have slightly different chemistry. Hyperspectral imaging can see those differences, so we can identify many different quality issues such as woody breast in chicken, fat/lean ratios, freshness and moisture content.

How can food processors use this information on composition and quality?

Pawluczyk: PPO’s Smart Imaging System uses an AI engine to collect and process the data from our imaging system; It ‘learns’ over time and gets even better at detection of FM or quality issues. It also means that PPO’s system can spot trends in your production. For example, using PPO’s technology, one of our clients was able to identify and correct an issue with their de-boning process, which helped them reduce customer charge-backs by 40%.

How confident can processors be that the system will catch the FM and quality issues they care about?

Pawluczyk: Part of PPO’s installation process with a new client is a very thorough testing process. Our team of experts works closely with our client to configure each system to the precise conditions of the plant and the products that are being processed. Using AI, our Smart Imaging System gathers and stores all this information, so it learns over time and is continuously improving.

What makes PPO’s Smart Imaging System different from other visual inspection systems on the market?

Pawluczyk: PPO’s is the only hyperspectral imaging system that is operating on the line in multiple plants across North America. It is being used in a variety of poultry and pork processing facilities and has proven to be highly effective in finding a wide range of foreign materials.

Ultimately, we think happy clients are the greatest proof that our system is working. We’re seeing repeat orders starting to come in from existing clients as they reap the cost benefits of improved detection in their plants.

Learn how food processors can leverage hyperspectral imaging on P&P Optica.

Content sponsored by P&P Optica.

Olga Pawluczyk, P&P OpticaAbout Olga Pawluczyk
President, CEO and Co-Founder
P&P Optica

Olga Pawluczyk is the co-founder and CEO of P&P Optica (PPO), based in Waterloo, Ontario, part of Canada’s largest and fastest growing tech community. Pawluczyk is an expert in medical imaging, with a technical background in systems engineering and deep knowledge of the science of spectroscopy. Under the leadership of Pawluczyk and her co-founder, her father Romek Pawluczyk, PPO launched in 2004 as a research company focused on developing high-end spectrometers. The company has evolved over the past eight years to focus on building solutions to the issues of safety and quality in the food processing industry. Pawluczyk is driven by the opportunity to combine emerging technologies to significantly improve the nutritional quality, safety, and sustainability of our food.

As a leader, Pawluczyk focuses on providing an engaging working environment to like-minded people who are excited to explore new challenges as part of the PPO team. Outside of PPO, She is active in the local tech community in Waterloo Region; is an avid reader who loves to discuss pretty much any topic over coffee (or wine); and enjoys spending time walking and biking.

Susanne Kuehne, Decernis
Food Fraud Quick Bites

Sergeant Pepper On Duty

By Susanne Kuehne
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Susanne Kuehne, Decernis
Pepper, food fraud
Find records of fraud such as those discussed in this column and more in the Food Fraud Database, owned and operated by Decernis, a Food Safety Tech advertiser. Image credit: Susanne Kuehne

A Northern Ireland-based analytical lab added white pepper to its portfolio of food authenticity tests based on spectroscopy with chemometric analysis. White pepper, the ripe berries of the piper nigrum plant, is undergoing an additional production step, fetches a higher price than black pepper and therefore is a target for fraudsters. Often, bulking substances like skins, flour, husks and spent materials are used, but in some cases of pepper fraud, the substances used were hazardous to human health.

Resource

  1. Taylor, P. (August 24, 2021). “With white pepper fraud on the up, Bia unveils authenticity test”. Securing Industry.
Susanne Kuehne, Decernis
Food Fraud Quick Bites

Things Do Not Get Better With Sage

By Susanne Kuehne
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Susanne Kuehne, Decernis
Sage, food fraud
Find records of fraud such as those discussed in this column and more in the Food Fraud Database. Image credit: Susanne Kuehne

Herbs remain a target for fraudsters. The latest investigation of sage samples by the Institute of Global Food Security (IGFS) at Queen’s University Belfast used a combination of spectroscopic and chemometric methods to check whether sage contained 100% of the actual herb. One quarter of samples from the UK included unapproved (fortunately, no hazardous) bulk material, such as tree leaves, some in significant concentrations of more than half of the product.

Resource

  1. Sage News”. (November 9, 2020). The Hippocratic Post.

 

Michael Bartholomeusz, TruTag
In the Food Lab

Intelligent Imaging and the Future of Food Safety

By Michael Bartholomeusz, Ph.D.
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Michael Bartholomeusz, TruTag

Traditional approaches to food safety no longer make the grade. It seems that stories of contaminated produce or foodborne illnesses dominate the headlines increasingly often. Some of the current safeguards set in place to protect consumers and ensure that companies are providing the freshest, safest food possible continue to fail across the world. Poorly regulated supply chains and food quality assurance breakdowns often sicken customers and result in recalls or lawsuits that cost money and damage reputations. The question is: What can be done to prevent these types of problems from occurring?

While outdated machinery and human vigilance continue to be the go-to solutions for these problems, cutting-edge intelligent imaging technology promises to eliminate the issues caused by old-fashioned processes that jeopardize consumer safety. This next generation of imaging will increase safety and quality by quickly and accurately detecting problems with food throughout the supply chain.

How Intelligent Imaging Works

In broad terms, intelligent imaging is hyperspectral imaging that uses cutting-edge hardware and software to help users establish better quality assurance markers. The hardware captures the image, and the software processes it to provide actionable data for users by combining the power of conventional spectroscopy with digital imaging.

Conventional machine vision systems generally lack the ability to effectively capture and relay details and nuances to users. Conversely, intelligent imaging technology utilizes superior capabilities in two major areas: Spectral and spatial resolution. Essentially, intelligent imaging systems employ a level of detail far beyond current industry-standard machinery. For example, an RGB camera can see only three colors: Red, green and blue. Hyperspectral imaging can detect between 300 and 600 real colors—that’s 100–200 times more colors than detected by standard RGB cameras.

Intelligent imaging can also be extended into the ultraviolet or infrared spectrum, providing additional details of the chemical and structural composition of food not observable in the visible spectrum. Hyperspectral imaging cameras do this by generating “data cubes.” These are pixels collected within an image that show subtle reflected color differences not observable by humans or conventional cameras. Once generated, these data cubes are classified, labeled and optimized using machine learning to better process information in the future.

Beyond spectral and spatial data, other rudimentary quality assurance systems pose their own distinct limitations. X-rays can be prohibitively expensive and are only focused on catching foreign objects. They are also difficult to calibrate and maintain. Metal detectors are more affordable, but generally only catch metals with strong magnetic fields like iron. Metals including copper and aluminum can slip through, as well as non-metal objects like plastics, wood and feces.

Finally, current quality assurance systems have a weakness that can change day-to-day: Human subjectivity. The people put in charge of monitoring in-line quality and food safety are indeed doing their best. However, the naked eye and human brain can be notoriously inconsistent. Perhaps a tired person at the end of a long shift misses a contaminant, or those working two separate shifts judge quality in slightly different ways, leading to divergent standards unbeknownst to both the food processor and the public.

Hyperspectral imaging can immediately provide tangible benefits for users, especially within the following quality assurance categories in the food supply chain:

Pathogen Detection

Pathogen detection is perhaps the biggest concern for both consumers and the food industry overall. Identifying and eliminating Salmonella, Listeria, and E.coli throughout the supply chain is a necessity. Obviously, failure to detect pathogens seriously compromises consumer safety. It also gravely damages the reputations of food brands while leading to recalls and lawsuits.

Current pathogen detection processes, including polymerase chain reaction (PCR), immunoassays and plating, involve complicated and costly sample preparation techniques that can take days to complete and create bottlenecks in the supply chain. These delays adversely impact operating cycles and increase inventory management costs. This is particularly significant for products with a short shelf life. Intelligent imaging technology provides a quick and accurate alternative, saving time and money while keeping customers healthy.

Characterizing Food Freshness

Consumers expect freshness, quality and consistency in their foods. As supply chains lengthen and become more complicated around the world, food spoilage has more opportunity to occur at any point throughout the production process, manifesting in reduced nutrient content and an overall loss of food freshness. Tainted meat products may also sicken consumers. All of these factors significantly affect market prices.

Sensory evaluation, chromatography and spectroscopy have all been used to assess food freshness. However, many spatial and spectral anomalies are missed by conventional tristimulus filter-based systems and each of these approaches has severe limitations from a reliability, cost or speed perspective. Additionally, none is capable of providing an economical inline measurement of freshness, and financial pressure to reduce costs can result in cut corners when these systems are in place. By harnessing meticulous data and providing real-time analysis, hyperspectral imaging mitigates or erases the above limiting factors by simultaneously evaluating color, moisture (dehydration) levels, fat content and protein levels, providing a reliable standardization of these measures.

Foreign Object Detection

The presence of plastics, metals, stones, allergens, glass, rubber, fecal matter, rodents, insect infestation and other foreign objects is a big quality assurance challenge for food processors. Failure to identify foreign objects can lead to major added costs including recalls, litigation and brand damage. As detailed above, automated options like X-rays and metal detectors can only identify certain foreign objects, leaving the rest to pass through untouched. Using superior spectral and spatial recognition capabilities, intelligent imaging technology can catch these objects and alert the appropriate employees or kickstart automated processes to fix the issue.

Mechanical Damage

Though it may not be put on the same level as pathogen detection, food freshness and foreign object detection, consumers put a premium on food uniformity, demanding high levels of consistency in everything from their apples to their zucchini. This can be especially difficult to ensure with agricultural products, where 10–40% of produce undergoes mechanical damage during processing. Increasingly complicated supply chains and progressively more automated production environments make delivering consistent quality more complicated than ever before.

Historically, machine vision systems and spectroscopy have been implemented to assist with damage detection, including bruising and cuts, in sorting facilities. However, these systems lack the spectral differentiation to effectively evaluate food and agricultural products in the stringent manner customers expect. Methods like spot spectroscopy require over-sampling to ensure that any detected aberrations are representative of the whole item. It’s a time-consuming process.

Intelligent imaging uses superior technology and machine learning to identify mechanical damage that’s not visible to humans or conventional machinery. For example, a potato may appear fine on the outside, but have extensive bruising beneath its skin. Hyperspectral imaging can find this bruising and decide whether the potato is too compromised to sell or within the parameters of acceptability.

Intelligent imaging can “see” what humans and older technology simply cannot. With the ability to be deployed at a number of locations within the food supply chain, it’s an adaptable technology with far-reaching applications. From drones measuring crop health in the field to inline or end-of-line positioning in processing facilities, there is the potential to take this beyond factory floors.

In the world of quality assurance, where a misdiagnosis can literally result in death, the additional spectral and spatial information provided by hyperspectral imaging can be utilized by food processors to provide important details regarding chemical and structural composition previously not discernible with rudimentary systems. When companies begin using intelligent imaging, it will yield important insights and add value as the food industry searches for reliable solutions to its most serious challenges. Intelligent imaging removes the subjectivity from food quality assurance, turning it into an objective endeavor.